Journal: International Journal of Pharmaceutics: X

Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition

doi: 10.1016/j.ijpx.2026.100489

Figure Lengend Snippet: In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots of Annexin V-FITC/PI staining for apoptosis analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

Article Snippet: After 24 h, the cells were harvested and stained with Annexin V-FITC/PI Cell Apoptosis Detection Kit (Beyotime, China) for flow cytometer analysis.

Techniques: In Vitro, Drug discovery, Incubation, MTT Assay, Flow Cytometry, Staining, Generated

Journal: Chemistry & Biodiversity

Article Title: Biochemical Characterization of a Kunitz‐Type Protease Inhibitor From Mimosa regnellii and Its Effects on Melanoma Cell Viability and Angiogenesis

doi: 10.1002/cbdv.202503760

Figure Lengend Snippet: Annexin V and propidium iodide under JTI action on melanoma cells. (A) Control cells 24 h; (B) cells treated with 0.65 µM of JTI for 24 h; (C) cells control 48 h; (D) cells treated with 0.65 µM of JTI for 48 h; (E) cells control 72 h; (F) cells treated with 0.65 µM of JTI for 72 h. FL2H‐ filter relating to propidium iodide. FL1H‐ filter relating to annexin V‐FITC. The results represent the average of three separate experiments.

Article Snippet: To evaluate the effects of JTI on cell death, the FITC/annexin V Apoptosis Kit with Dead Cell Annexin FITC (fluorescein isothiocyanate) and PI (propidium iodide), for flow cytometry (Invitrogen, Catalog No. V13242) was used.

Techniques: Control

Journal: International Journal of Nanomedicine

Article Title: Spatholobus suberectus Stem-Derived Extracellular Vesicle-Like Particles Attenuate Glucocorticoid-Induced Osteoporosis via NRF2/HO-1 Signaling

doi: 10.2147/IJN.S585928

Figure Lengend Snippet: SS-EVLP attenuates dexamethasone-induced loss of viability and apoptosis in MC3T3-E1 cells. ( a and d ) Effects of different concentrations of SS-EVLP on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( b and e ) Effects of different concentrations of dexamethasone (DEX) on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( c and f ) SS-EVLP-mediated restoration of cell viability in MC3T3-E1 cells exposed to 100 μM DEX at 24 and 48 h, respectively. ( g ) Representative flow cytometric plots of Annexin V-FITC/PI double staining in the indicated groups. ( h ) Quantification of apoptotic cells. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Cell Counting Kit-8 (CCK-8) and Annexin V-FITC/PI apoptosis detection kits were purchased from Beyotime (Shanghai, China).

Techniques: Double Staining